Figure 4.

Effect of DSF and/or JQ-1 on oxidative stress and cuproptosis of A549 cells. Levels of (A) ROS, (B) MDA and (C) SOD in A549 cells treated with DSF/Cu²⁺ and/or JQ-1 were detected by commercial assay kits. (D) Expression of cuproptosis-associated proteins in A549 cells treated with DSF/Cu²⁺ and/or JQ-1 was detected by western blot. ^***P<0.001 vs. A549. ^#P<0.05 and ^###P<0.001 vs. A549 + DSF/Cu²⁺. DSF, disulfiram; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase; FDX1, ferredoxin-1; ATP7B, ATPase copper-transporting β; SLC31A1, solute carrier family 31 member 1; SDHB, succinate dehydrogenase B; PD-L1, programmed death-ligand 1; HIF-1A, hypoxia inducible factor-1A.