FIG. 2.

Coelution of p₄A and palmitoyl-CoA synthetic activities upon gel filtration. A sample of acyl-CoA synthetase was applied to a Bio-Sil-Sec 250 column as described in Materials and Methods (inset); the arrow marks the column void volume; peaks p and a correspond to protein and adenine nucleotides, respectively. The activities of synthesis of p₄A (•) and palmitoyl-CoA (○) were studied with 15 and 0.33 μl of the column fractions, respectively; [2,8-³H]ATP was used as radioactive substrate. Other conditions were as described in Materials and Methods. The broken line represents absorbance at 280 nm.