Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of asymmetrical dinucleoside tetraphosphatase on Ap4A (left panel) or Ap5A (right panel) obtained from ATP or p4A, catalyzed by acyl-CoA synthetase. Reaction mixtures containing 50 mM Tris-HCl (pH 7.5), 1 mM MgCl2, and purified (see Materials and Methods) Ap4A or Ap5A (33 μM) were treated with asymmetrical dinucleoside tetraphosphatase (0.4 or 0.7 mU/ml, respectively). At the times indicated, aliquots were taken and analyzed by HPLC.
