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. 2023 Dec 20;625(7994):385–392. doi: 10.1038/s41586-023-06857-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, Relative body weight change of 8–12-week-old Dars2^fl/fl and Dars2^tamIEC-KO mice after tamoxifen administration (n = 21 per genotype). b, Immunoblot analysis with the indicated antibodies of protein extracts from SI IECs of Dars2^fl/fl and Dars2^tamIEC-KO mice 7 days after the last tamoxifen injection (n = 6 per genotype). β-actin was used as the loading control. c, Representative TEM micrographs of proximal SI sections and quantification of the mitochondria integrity distribution as a percentage of normal, partly affected and damaged mitochondria based on the electron density and cristae morphology in Dars2^fl/fl mice (n = 4 mice, n = 663 mitochondria in n = 69 IECs) and Dars2^tamIEC-KO mice (n = 4 mice, n = 707 mitochondria in n = 80 IECs) 7 days after tamoxifen. d, Representative images of sections from the proximal SI of Dars2^tamIEC-KO and Dars2^fl/fl mice stained with H&E, COX and SDH or immunostained with Ki67. e, Representative images of proximal and distal SI sections of Dars2^fl/fl and Dars2^tamIEC-KO mice stained with ORO or immunostained with PLIN2. f, TAG content in proximal SI of Dars2^fl/fl and Dars2^tamIEC-KO mice (n = 8 per genotype). g, Concentration of glucose, total cholesterol and TAGs in sera from Dars2^fl/fl mice (n = 9 (glucose, total cholesterol), n = 8 (TAG)) and Dars2^tamIEC-KO mice (n = 11 (glucose, total cholesterol), n = 7 (TAG)) 7 days after the last tamoxifen injection. In c, f and g, dots represent individual mice, bar graphs show the mean ± s.e.m. and P values were calculated using two-way analysis of variance (ANOVA) with Bonferroni’s correction for multiple comparison (a), two-sided chi-square test (c) or two-sided nonparametric Mann–Whitney U-test (f,g). In d,e, histological images are representative of the number of mice analysed as indicated in Supplementary Table 4. In b, each lane represents one mouse. Scale bars, 1 μm (c) or 50 μm (d,e). For gel source data, see Supplementary Fig. 1.

Source Data