Overview of the dapDE locus of C. glutamicum. The thick black line shows the general arrangement with selected restriction sites. Selected fragments used in this study are shown over the line, and the regions used to construct specific mutants, either by gene disruption (::) or gene deletion (▵), are shown under the line. The scale at the bottom of the figure shows length (in base pairs). Abbreviations: B, BamHI; Bg, BglII; E, EcoRI; H, HindII; Hi, HindIII; K, KpnI; P, PstI; Sa, SacI; Sc, ScaI; Sg, SgrAI; Sl, SalI; X, XhoI.