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Growth of two strains of C. glutamicum. The wild-type strain (open symbols) and the dapD mutant of C. glutamicum (solid symbols) are shown. Cells were grown in rich BHI medium plus 4% glucose without (NH4)2SO4 (circles), with 20 mM (NH4)2SO4 (squares), and with 100 mM (NH4)2SO4 (triangles). Growth was measured by monitoring A600. In addition, the decrease in the concentration of free ammonium (×) for the dapD mutant without (NH4)2SO4 is shown.
