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. 2024 Jan 11;15:451. doi: 10.1038/s41467-024-44689-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Flow cytometry analysis of Mitotracker green in cell populations from blood and intestine of healthy humans. Lines in the dot plot show mean values and graph shows data from 2 to 5 independent experiments. b Mitotracker green in cell populations isolated from mLN and intestine of C57BL/6J mice. Lines in the dot plot show mean values and data show n = 3 biological replicates over nine independent experiments. c Western blot of Tom20 and TFAM. The data shown are representative of six independent experiments. d Mitotracker green in antigen-specific and polyclonal cell populations isolated from mLN and intestine of mice orally challenged with LmOVA and analyzed 7 days post-infection. Lines in the dot plot show mean values and data show n = 5 biological replicates over four independent experiments. e TMRM staining in the indicated cell populations. Lines in the dot plot show mean values and data show n = 3 biological replicates over five independent experiments. f TMRM in antigen-specific and polyclonal cells isolated from mLN and intestine of mice orally challenged with LmOVA and analyzed 7 days post-infection. Lines in the dot plot show mean values and data show n = 5 biological replicates over four independent experiments. g TMRM staining in cells upon treatment with oligomycin and FCCP. TMRM was used at a concentration of 10 nM. TMRM values are shown normalized to the baseline of staining in the respective population. Data are representative of three independent experiments. h Mass spectrometry analysis of AMP, ADP, ATP, and ATP:AMP ratio. The bars show mean ± SEM. Data show three independent experiments analyzed simultaneously. i Intracellular interferon-γ (IFN-γ) protein expression in cells isolated from mLN and intestine of GREAT mice. Lines in the dot plots show mean values and data show n = 3 biological replicates over two independent experiments. a, b, d–f, and h Statistics were performed using one-way ANOVA and Tukey’s multiple comparison correction. Source data are provided as a Source Data file.