TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| V. parahaemolyticus | ||
| BB22TR | Wild type; translucent variant | R. Belas (7) |
| BB22OP | Wild type; opaque variant | BB22TR (this work) |
| LM1017 | lfgE313::lux (formerly lafX313::lux) | RS313 (22) |
| LM4449 | Opaque phenotype; BB22TR/pLM1950 | BB22TR (this work) |
| LM4437 | Translucent phenotype; opaR::Tn5 | BB22TR (this work) |
| LM4462 | Translucent phenotype; opaR::Tn5 | BB22OP (this work) |
| LM4565 | IPTG-inducible opaque phenotype; LM4462/pLM2035 | LM4462 (this work) |
| LM4572 | Translucent phenotype; LM4462/pLM1835 | LM4462 (this work) |
| LM4582 | Translucent phenotype; BB22TR/pLAFRII | BB22TR (this work) |
| E. coli | ||
| DH5α | F−endA1 hsdR17 (rK−) supE44 thi-1 λ− recA1 deoR gyrA96 relA1 Δ(argF-lacZYA)U169 φ80dlacZΔM15 | Bethesda Research Laboratories |
| LLM1956 | DH5α/pRS205 (dark) | This work |
| LLM1957 | DH5α/pRS205/pLM1950 (bright) | This work |
| LLM1958 | DH5α/pRS205/pLM1952 (dark) | This work |
| Plasmids | ||
| pLAFRII | Tetr broad-host-range cosmid vector | 11 |
| pRS205 | Apr; luxCDABE+ operon (V. harveyi) | R. Showalter (29) |
| pLM1835 | Camr Apr broad-host-range expression vector carrying lacIq and Ptac | pMMB66EH (12, 33) |
| pLM1950 | TetropaR+ | pLAFRII V. parahaemolyticus bank (21) |
| pLM1952 | Tetr KanropaR294::Tn5 | pLM1950 (this work) |
| pLM1980 | Apr; pUC19 with 2.0-kb PstI fragment from pLM1952 containing part of Tn5 insertion in opaR | pUC19 and pLM1952 |
| pLM2035 | Camr ApropaR+ under Ptac control | pLM1835 (this work) |