Skip to main content
. 2024 Jan 10;15:447. doi: 10.1038/s41467-024-44732-2

Fig. 2. DJ1 KO midbrain organoids have increased protein glycation.

Fig. 2

a Dot blots for MGH protein modification and actin (ACTB)/Ponceau loading control for CTR and DJ1 KO iPSCs (n = 3), day 40 (n = 6), 100 (n = 5), and 200 (n = 5) midbrain organoids (Two-tailed t-test was used for mean comparisons). b Immunoblots for flRAGE, for CTR and DJ1 KO day 40 (n = 3) and day 200 (n = 6) and sRAGE CTR and DJ1 KO day 40 (n = 3) and day 200 (n = 6) in midbrain organoids; actin (ACTB) was used as loading control (Two-tailed t-test was used for mean comparisons). c Dot blots for MGH protein modification and actin (ACTB) loading control in vehicle (Veh) or aminoguanidine (Amino) treated CTR and DJ1 KO day 100 midbrain organoids (n = 3, Two-way ANOVA followed by Tukey’s for the multiple comparisons test). d Immunoblots for phospho-α-syn (S129) and actin (ACTB) loading control in vehicle (Veh) or aminoguanidine (Amino) treated CTR and DJ1 KO day 200 midbrain organoids (n = 3, Two-way ANOVA followed by Bonferroni’s for the multiple comparisons test). All data are represented in mean ± S.E.M, data points are individual well differentiation, and the p-value was reported on the graph highlighted comparison. All measurements were taken from distinct samples.