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. 2023 Nov 23;31(1):40–52. doi: 10.1038/s41418-023-01237-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a NTD-DmrB cells were preloaded with green Dextran beads and live cell imaging was recorded following dimerizer (D) and Z-VAD-FMK (Z) treatment. Scale bar, 10 μm. b NTD-DmrB cells were treated with DMSO or D/Z for 2 h and stained with antibodies against FLAG and LAMP1. Scale bar, 10 μm. c Lysosomal membrane protein TMEM192 with 3×HA tags at C-terminus was stably expressed in NTD-DmrB cells. Cells were treated with DMSO or D/Z for 2 h and lysosomes were precipitated with anti-HA magnetic beads and Western blotting was performed with the indicated antibodies. Control sample was from DMSO-treated parental NTD-DmrB cells. LAMP1, lysosome marker; EGFR, plasma membrane marker; GGA1, Golgi marker; PMP70, peroxisome marker; Tom40, mitochondria marker; CALR (Calreticulin), ER marker. Immunoprecitated lysosomes were analyzed with non-reducing SDS-PAGE (d) or SDD-AGE (e) and probed with an anti-FLAG antibody. f Working model. Upon D/Z treatment, NTD-DmrB tetramers form polymers on lysosomal membrane and induces LMP.