FIG. 3.

Expression of the proteins HcnA, HcnB, and HcnC′. The hcnABC′ genes were cloned in the T7 expression vector pEB16, giving plasmids pME3210 (hcnABC′) and pME3209 (hcnC′) (Fig. 1). Cultures of P. aeruginosa ADD1976 carrying either of these plasmids were induced with IPTG (2 mM) (+) or not induced (−). The proteins were labeled with [³⁵S]methionine, separated by electrophoresis in an SDS–15% polyacrylamide gel, and visualized by autoradiography.