TABLE 2.
HCN biosynthesis in P. fluorescens CHA0 and E. coli BL21 harboring hcnABC recombinant plasmids
| Strain | Relevant genotype | HCN productiona (μM) |
|---|---|---|
| CHA0 | hcnABC+ | 89 ± 5 |
| CHA77 | Δhcn | <5 |
| CHA77/pME3013 | hcnABC+ | 96 ± 32 |
| CHA77/pME3206 | PKm-hcnABC+ | 855 ± 90 |
| BL21/pEB16 | <5 | |
| BL21/pME3210 | PT7-hcnABC′ | 132 ± 25 |
| BL21/pME3212 | PT7-hcnABC+ | 102 ± 13 |
a
P. fluorescens was grown in MMC with O2 limitation and E. coli was grown in a rich, glucose-containing medium as indicated in Materials and Methods. Mean ± standard deviation values for triplicate experiments are given.