Fig. 3. ADSCs-mito transplantation increased smooth muscle content in corpus cavernosum. (A) Immunofluorescence staining of SMA in penile tissues of sham, PBS, ADSCs-mito and ADSCs groups, scale bar, 50 µm. (B) Quantitative analysis of the SMA immunofluorescence positive area in the four groups. (C, D) The expression of SMA protein in penile tissues from the four groups was determined by western blotting. (E) Masson’s trichrome staining of the penile tissues used to assess the smooth muscle/collagen ratio in the four groups, smooth muscle and collagen were stained red and blue, respectively, scale bar, 500 µm and 200 µm. (F) Quantitative analysis of the Masson’s trichrome staining results. Error bars: mean±standard deviation. ADSCs: adipose derived mesenchymal stem cells, ADSCs-mito: ADSCs-derived mitochondria, Mito: mitochondria, SMA: smooth muscle actin, PBS: phosphate buffer solution. ^**p<0.01, ^***p<0.001 comparison with PBS group.