Fig. 4. ADSCs-mito transplantation attenuated oxidative stress and apoptosis in corpus cavernosum. (A, C) Immunofluorescent staining of ROS in penile tissue from the sham, PBS, ADSCs-mito, and ADSCs groups, scale bar, 50 µm. (B, D) Immunofluorescent staining of mtROS in penile tissue from the four groups, scale bar, 50 µm. (E) The superoxide dismutase levels in penile tissues were detected in the four groups. (F, G) The expression of cleaved-caspase 3 protein in penile tissues from the four groups was determined by western blot. (H) The adenosine triphosphate levels in penile tissues were detected in four groups. Error bars: mean±standard deviation. ADSCs: adipose derived mesenchymal stem cells, ADSCs-mito: ADSCs-derived mitochondria, Mito: mitochondria, ROS: reactive oxygen species, mtROS: mitochondria derived reactive oxygen species, PBS: phosphate buffer solution. ^*p<0.05, ^**p<0.01, ^***p<0.001, ^****p<0.0001 comparison with the PBS group.