TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or phenotype | Reference or source |
|---|---|---|
| Strains | ||
| Bacillus subtilis | ||
| 168 | trpC2 (wild type) | BGSC,a 1A1 |
| MD186 | trpC2 glck::spc | This workb |
| Escherichia coli | ||
| JM101 | supE thi Δ(lac-proAB) F′ [traD36 proAB+ lacIqlacZΔM15] | 20 |
| RB791 | F′ [lacIqL8] hsdR+ hsdM | 4 |
| UE26 | F−ptsG2 ptsM1 glk7 rpsL150 | 3, 5 |
| Plasmids | ||
| pDG1726 | pSB119 derivative containing the spc antibiotic cassette | 13 |
| pMD402 | pUC18 derivative containing degS under degQ36 promoter control | 6 |
| pMD492 | pMD402 derivative containing glcK under degQ36 promoter control | This workb |
| pMD495 | pMD492 derivative carrying glcK::spc | This workb |
| pMD496 | pQE9 derivative carrying glcK fused in frame to the His tag-coding region | This workb |
| pQE9 | Expression vector for His tag fusions under T5 promoter control | 24 |
a
BGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus, Ohio.
b
For detailed description of construction, see text.