Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 10;22:43. doi: 10.1186/s12967-023-04830-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — PF-543 inhibits SphK1 activity in DEN-treated mice. A Total and phospho-SphK1 levels were examined using immunohistochemical staining in tumorous (T) and para-tumorous (para-T) tissues of human HCC specimens; scale bar = 50 μm; n = 10. B Schematic illustration of treatment schedules for in vivo studies, created with BioRender.com. C Body weight over 12 weeks of vehicle (veh) or PF-543 (PF) treatment. D Liver mass was weighed. E phospho(p)-SphK1 SphK1 and SphK2 protein levels in liver tissues were determined using Western blotting. F Hepatic levels of ceramide (Cer), sphingosine (Sph), and S1P were analyzed using lipidomics. C-F n = 9. Data are expressed as mean ± SD. *p < 0.05; ***p < 0.001