Fig. 1.

Workflow for mitosis annotation. a H&E and aligned PHH3 WSIs were prepared. b H&E and PHH3 image tiles with a size of one HPF were cut from corresponding WSIs. c Two pathology residents annotated mitoses on H&E-PHH3 HPF tiles independently using d the mitosis annotation interface. e A neuropathologist reviewed mitosis annotations from the two pathology residents and provided the final judgment for each one. f Mitosis annotations were transferred onto H&E WSIs for further analysis (each dot represents a mitosis annotation)