Fig 3.
NTXR diminishes ability of E. coli to cause an infection in zebrafish larvae. Zebrafish larvae were infected through the caudal vein 1 day post-fertilization (dpf), and survival was monitored until 4 days post-infection (dpi). Log phase cultures of respective strains were used to prepare infection solutions and inoculum was determined via injection into PBS followed by a viable cell count. Larvae injected with phenol red/PBS (1/1, vol/vol) served as controls (data not shown). Each line represents survival of 10–20 larvae per condition (WT 400 CFU, n = 30).