Fig 4.

Effect of thiostrepton on gastric cancer cell growth and Foxm1 expression. Percent epithelial cell confluence of (A) MKN45 and (B) AGS gastric cancer cell lines after 72 hours following incubation with vehicle control or escalating concentrations of thiostrepton. Percent cell confluence was measured using the MTT assay. AGS gastric cancer cells incubated with thiostrepton at 0, 0.5, and 1 µM and incubated (C) with H. pylori strain SS1 or (D) uninfected at an MOI of 1:100 or vehicle control (DMSO). Following incubation at 37°C for 24 hours, fold changes of Foxm1 compared to the housekeeping gene Gapdh were determined by qPCR. Thiostrepton inhibited the proliferation of gastric cancer cell lines in a dose-dependent manner and decreased Foxm1 expression in H. pylori-infected and uninfected AGS cells. *P < 0.05, **P < 0.01.