Fig 5.

Double FISH analyses revealed colocalization of Smgpcr20, Smnpp26, and Smnpp40. Results of double FISH experiments providing first hints for Smgpcr20 and Smnpp26 transcript colocalization in neuronal cells within the head region and along the body of sF. For counterstaining, cells were labeled by Hoechst 33342 (blue). FITC-labeled Smgpcr20 transcripts are shown in green, and DIG-labeled Smnpp26 transcripts are shown in magenta. (A) Results of double FISH experiments showing Smgpcr20 and Smnpp26 transcript colocalization in the head and body regions of sF. (B) Results of double FISH experiments showing Smgpcr20 and Smnpp40 transcript colocalization in the head and body regions of sF. Sense probes of each gene served as negative controls and showed no signals (C). Cells were stained Hoechst 33342 (blue), FITC-labeled Smgpcr20 transcripts are shown in green, and DIG-labeled Smnpp40 transcripts are shown in red. Scale bars: 50 µm; right panel: 20 µm.