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. 2023 Dec 4;12(1):e02403-23. doi: 10.1128/spectrum.02403-23

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Copyright © 2023 Song et al.

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Fig 1 — Construction and identification of rAd5-PEDV-S. (A) Ad-related cytopathic effect (CPE) caused by rAd5-PEDV-S in HEK293 cells. The left figure represents CPE caused by rAd5-PEDV-S, right represents normal cells. (B) The genetic stability of rAd5-PEDV-S in HEK293 cells was detected by PCR, “P” means passage. “+” means positive control, “-” means negative control. (C) Western blotting analysis of S protein expressed by rAd5-PEDV-S in HEK293 cells. (D)Immunofluorescence assay (IFA) analysis of S protein expressed by rAd5-PEDV-S in HEK293 cells.