Fig 1.

B. theta is more sensitive to deconjugated bile acids which are able to alter the membrane integrity. (A) Example of bile acid transformation carried out by BSHb and HSDHa. (B) WT and the triple KO strains of B. theta were used to determine MICs in different bile acids. Bars represent mean MICs (n = 2–3 biological replicates, n = 2 technical replicates). Error bars represent standard error. Asterisks denote significant (P < 0.05) by Mann-Whitney between strains. (C) WT and the triple KO strains were incubated with different bile acids and stained with propidium iodide. Asterisks denote significant (P < 0.0001) differences between strains by two-way ANOVA with Šídák’s multiple comparisons test.