Fig 4.

Global metabolism of B. theta is impacted by the absence of bile acid-altering enzymes in nutrient-limited conditions. (A) Volcano plot of differentially expressed genes identified by RNASeq during nutrient-limiting conditions. Points are colored when P < 0.05 by Wald test with Benjamini and Hochberg correction and log₂fold change is >2 (red) or <2 (green). The number of significant genes is listed in the top corners for both directions. (B) KEGG orthologs detected for genes listed in (A). (C) Heatmap of differentially expressed genes present in the top four KEGG orthology categories in (B).