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Limit of detection of the qPCR assay for L. plantarum ATCC 202195. Cycle quantification (Cq) numbers were plotted against the log10 starting quantity cell numbers from two sets of qPCR experiments, which are outlined in Table 3. The reaction efficiency was calculated to be 97.8% using the following equation, 10(–1/slope) – 1, where the slope was equal to −3.3758, as determined from a linear regression model. The gray shaded area represents the 95% CI of the slope of the standard curve.
