TABLE 1.
Target | Primer/probe name | Sequence (5′ to 3′) |
---|---|---|
Lactiplantibacillus plantarum ATCC 202195 | LP202195-F (forward) | GAG CAG ATT ATT GGC GAT TGG |
LP202195-R (reverse) | CAT CTA GCG ATA ACG TTC CTT G | |
LP202195-P (probe) | 6FAM/GCC TTT GTA TAC GAC GTT CAT TTA GCT AGT C/MGB-NFQ a , b |
6FAM = 5′6-FAMTM reporter.
MGB-NFQ = 3′minor groove binder–non-fluorescent quencher.
The expected amplicon length was 169 bp, and the probe length was 31 bp.