TABLE 3.

Limit of detection of the qPCR assay for L. plantarum ATCC 202195 ^a

Experiment number	Cell number	Replicates run	Log (cell number)	Cq mean	Cq standard deviation	Proportion of reactions that amplified (%)	Mass of DNA input per μL
1	1.57E + 07	3	7.196	18.487	0.919	100	50 ng
	1.57E + 06	3	6.196	21.461	0.320	100	5 ng
	1.57E + 05	3	5.196	24.625	0.519	100	0.5 ng
	1.57E + 04	3	4.196	27.964	0.634	100	0.05 ng
	1,570	3	3.196	31.517	0.188	100	0.005 ng
	157	3	2.196	35.09	0.186	100	500 fg
	15.7	3	1.196	39.144	0.464	100	50 fg
	1.57	3	0.196	NaN	NaN	0	5 fg
	0.157	3	0.804	NaN	NaN	0	0.5 fg
2	157	16	2.196	35.197	0.249	100	500 fg
	78.5	16	1.895	36.036	0.442	100	250 fg
	39.25	16	1.594	36.999	0.276	100	125 fg
	19.63	16	1.293	38.029	0.377	100	62.5 fg
	9.81	16	0.992	38.999	0.696	75	31.25 fg
	4.9	16	0.69	NaN	NaN	0	15.625 fg

^a Cq, cycle quantification number; NaN, not a number.