TABLE 6.
Recovery of L. plantarum ATCC 202195 gDNA from stool samples spiked with L. plantarum ATCC 202195 cells b
| Sample description | Expected log starting quantity cells | Observed log starting quantity cells, mean (SD) a | Observed log starting quantity minus expected log starting quantity | Percent recovery (observed log starting quantity divided by expected log starting quantity) | Expected Cq value | Observed Cq value, mean (SD) a | Observed Cq value minus expected Cq value |
|---|---|---|---|---|---|---|---|
| Stool sample 1 (100 mg) plus 9.42 × 108 L. plantarum ATCC 202195 cells | 7.37 | 7.28 (0.11) | 0.10 | 98.8 | 20.39 | 20.71 (0.38) | −0.32 |
| Stool sample 2 (100 mg) plus 9.42 × 108 L. plantarum ATCC 202195 cells | 7.37 | 7.41 (0.09) | −0.04 | 100.5 | 20.39 | 20.24 (0.30) | 0.14 |
| Stool sample 3 (100 mg) plus 9.42 × 108 L. plantarum ATCC 202195 cells | 7.37 | 7.36 (0.00) | 0.01 | 99.9 | 20.39 | 20.43 (0.02) | −0.04 |
| Stool sample 4 (100 mg) plus 9.42 × 108 L. plantarum ATCC 202195 cells | 7.37 | 7.32 (0.07) | 0.05 | 99.3 | 20.39 | 20.55 (0.25) | −0.17 |
| Stool sample 5 (100 mg) plus 9.42 × 108 L. plantarum ATCC 202195 cells | 7.37 | 7.13 (0.01) | 0.24 | 96.7 | 20.39 | 21.19 (0.03) | −0.80 |
| Stool sample 1 (100 mg) plus 9.42 × 107 L. plantarum ATCC 202195 cells | 6.37 | 7.04 (0.01) | −0.67 | 110.5 | 23.78 | 21.50 (0.02) | 2.28 |
| Stool sample 2 (100 mg) plus 9.42 × 107 L. plantarum ATCC 202195 cells | 6.37 | 6.65 (0.02) | −0.28 | 104.4 | 23.78 | 22.83 (0.08) | 0.94 |
| Stool sample 3 (100 mg) plus 9.42 × 107 L. plantarum ATCC 202195 cells | 6.37 | 6.44 (0.09) | −0.07 | 101.1 | 23.78 | 23.54 (0.29) | 0.24 |
| Stool sample 4 (100 mg) plus 9.42 × 107 L. plantarum ATCC 202195 cells | 6.37 | 6.31 (0.03) | 0.07 | 99.1 | 23.78 | 24.00 (0.09) | −0.22 |
| Stool sample 5 (100 mg) plus 9.42 × 107 L. plantarum ATCC 202195 cells | 6.37 | 6.42 (0.05) | −0.05 | 100.8 | 23.78 | 23.60 (0.18) | 0.18 |
| Stool sample 1 (100 mg) plus 9.42 × 106 L. plantarum ATCC 202195 cells | 5.37 | 5.56 (0.12) | −0.19 | 103.5 | 27.17 | 26.54 (0.41) | 0.63 |
| Stool sample 2 (100 mg) plus 9.42 × 106 L. plantarum ATCC 202195 cells | 5.37 | 5.51 (0.03) | −0.14 | 102.6 | 27.17 | 26.69 (0.09) | 0.48 |
| Stool sample 3 (100 mg) plus 9.42 × 106 L. plantarum ATCC 202195 cells | 5.37 | 5.11 (0.03) | 0.26 | 95.2 | 27.17 | 28.05 (0.11) | −0.88 |
| Stool sample 4 (100 mg) plus 9.42 × 106 L. plantarum ATCC 202195 cells | 5.37 | 5.14 (0.01) | 0.23 | 95.7 | 27.17 | 27.95 (0.04) | −0.78 |
| Stool sample 5 (100 mg) plus 9.42 × 106 L. plantarum ATCC 202195 cells | 5.37 | 5.28 (0.01) | 0.09 | 98.3 | 27.17 | 27.47 (0.04) | −0.30 |
| Stool sample 1 (100 mg) with no L. plantarum ATCC 202195 cells added | c - | - | - | - | - | - | - |
| Stool sample 2 (100 mg) with no L. plantarum ATCC 202195 cells added | - | - | - | - | - | - | - |
| Stool sample 3 (100 mg) with no L. plantarum ATCC 202195 cells added | - | - | - | - | - | - | - |
| Stool sample 4 (100 mg) with no L. plantarum ATCC 202195 cells added | - | - | - | - | - | - | - |
| Stool sample 5 (100 mg) with no L. plantarum ATCC 202195 cells added | - | - | - | - | - | - | - |
a
Mean and SD between two replicates.
b
Cq, cycle quantification number.
c
"-” signifies that data are not applicable in a given cell.