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. 2024 Jan 10;223(3):e202307147. doi: 10.1083/jcb.202307147

Figure 2.

Figure 2.

Cby3−/− males are infertile with sharply kinked sperm tails. (A) Quantification of testis weights (n = 6 mice per genotype). The average weight of both testes is plotted. (B) Sperm counts (n = 4 mice per genotype). (C) Quantification of sperm with kinked tails (n = 5 mice per genotype). (D) Representative DIC images of cauda epididymal sperm. Yellow arrows point to the direction of sperm movement. Scale bar, 10 μm. (E and F) Sperm velocity and hyperactivation were measured under non-capacitating (NC) and capacitating (CAP) conditions using CASA (n = 11–13 mice per genotype). (G) Quantification of Western blot results for PKA substrate phosphorylation (pPKAs) and tyrosine phosphorylation (pY) under NC and CAP conditions (n = 11–13 mice per genotype). See also Fig. S3 H. Means ± SD are shown. Student’s t test: *P < 0.05, **P < 0.01, ***P < 0.001, not significant (ns).