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. 2024 Jan 10;223(3):e202307147. doi: 10.1083/jcb.202307147

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© 2024 Hoque et al.

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Figure S4. — Generation and initial characterization of ciBAR1^−/− mice. (A) Schematic of ciBAR1 protein structure. (B) Generation of ciBAR1^−/− mice. (C) PCR genotyping of ciBAR1 mice. The locations of genotyping primers for WT (P1 and P2, 650 bp) and KO (P3 and P4, 586 bp) alleles are indicated by arrows in B. (D) Testis lysates prepared from adult mice were processed for Western blotting using antibodies against ciBAR1 and loading control GAPDH. (E) Representative adult testis images. (F) Representative images of PAS staining of adult testis sections. (G) Paraffin sections of male efferent ducts were immunostained for A-tub. Nuclei were detected with DAPI. Scale bars for E, 5 mm; F, 100 µm; and G, 50 µm. Source data are available for this figure: SourceData FS4.