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. 2024 Jan 5;223(3):e202306013. doi: 10.1083/jcb.202306013

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© 2024 Endo et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 2. — Endosomal stress induces the expression of immune-related genes. (A) Total cell lysates from HeLa cells transfected with the indicated siRNAs were immunoblotted with the indicated antibodies. (B) Experimental procedures are shown (see also Materials and methods section). HeLa cells transfected with the indicated siRNAs were subjected to LC-MS/MS analysis. (C) The mean Log₂ FC (siUSP8/siControl, and siTSG101/siControl) are shown on the x- and y-axis, respectively. (D) The bubble plot illustrating the GO enrichment test for biological process of proteins increased in USP8-depleted cells is shown (top 10 categories). (E) The heatmap illustrates the changes of interferon-induced proteins increased in USP8-depleted cells. Colors indicate the scaled intensity. (F) Total cell lysates from HeLa cells transfected with the indicated siRNAs were immunoblotted with the indicated antibodies. Source data are available for this figure: SourceData F2.