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. 2024 Jan 5;223(3):e202306013. doi: 10.1083/jcb.202306013

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© 2024 Endo et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S4. — Accumulation of phosphorylated p62 on endosomes in USP8-depleted cells. (A) Total cell lysates from HeLa cells transfected with the indicated siRNAs were immunoblotted with the indicated antibodies. (B and C) HeLa cells transfected with the indicated siRNAs were immunostained with the indicated antibodies and DAPI. Scale bar, 20 μm. (D) Parental HeLa cells and HeLa cells stably expressing siRNA-resistant Myc-USP8 WT and C786A were transfected with the indicated siRNAs. Total cell lysates from cells were immunoblotted with the indicated antibodies. (E and F) HeLa cells stably expressing siRNA-resistant Myc-USP8 WT and C786A were transfected with the indicated siRNAs. Cells were immunostained with the indicated antibodies and DAPI. Scale bar, 20 μm. Source data are available for this figure: SourceData FS4.