Table 1.

The half-lives of nucleic acids against various nucleases either in aqueous buffer or in 2 M CDHP

Nuclease	Half-life in water	Half-life in 2 M CDHPd	Relative increase in half-life
RNase Aa	<5 s	224.3 days	>3 875 904
RNase Hb	37.9 s	267.6 days	610 043
Exonuclease Ta (RNase T)	8.4 s	636.1 days	6 542 742
DNase Ic	6.0 s	229.3 days	3 301 920
T5c exonuclease	56.0 s	730.2 days	1 126 594
Exonuclease Vc	144.4 s	304.6 days	182 253
S1 nucleasea	7.5 s	158.7 days	1 828 224

^aMG aptamer-FAM was employed. ^bMnSOD mRNA-22-Cy5, hybridized with MnSOD MB, was employed. ^cMnSOD MB-Cy3 was employed. ^dAssuming the first order decay kinetics, half-lives of RNAs and DNAs are calculated based on Supplementary Figure S13. The relative increase in half-life is the half-life of nucleic acids in 2 M CDHP divided by that in water.