Table 2.
Intra-assay coefficient of variation (CV) of rNTP standard samples
| rNTP | Input (pmol) | Mean (%) | SD (%) |
|---|---|---|---|
| ATP | 125 | 0.9 | 0.2 |
| ATP | 62.5 | 1.4 | 1.0 |
| ATP | 31.3 | 1.5 | 0.4 |
| ATP | 15.6 | 2.8 | 1.5 |
| ATP | 7.81 | 4.5 | 0.6 |
| GTP | 125 | 1.5 | 0.6 |
| GTP | 62.5 | 2.3 | 1.2 |
| GTP | 31.3 | 1.8 | 1.6 |
| GTP | 15.6 | 1.6 | 0.5 |
| GTP | 7.81 | 5.6 | 2.2 |
| UTP | 125 | 1.4 | 1.1 |
| UTP | 62.5 | 1.8 | 0.6 |
| UTP | 31.3 | 3.5 | 4.0 |
| UTP | 15.6 | 2.4 | 1.5 |
| UTP | 7.81 | 2.4 | 2.5 |
| CTP | 125 | 2.5 | 1.7 |
| CTP | 62.5 | 1.9 | 1.6 |
| CTP | 31.3 | 1.2 | 0.5 |
| CTP | 15.6 | 1.4 | 1.1 |
| CTP | 7.81 | 1.5 | 0.5 |
| CTP | 3.91 | 3.6 | 2.4 |
The mean and SD represent CVs from three independent triplicate measurements. The reaction volumes were 10 μl (5 μl sample and 5 μl assay reagent).