Table 3.
Intra-assay coefficient of variation (CV) of liver extracts
| Nucleotides | Mean (%) | SD (%) |
|---|---|---|
| ATP | 1.7 | 0.9 |
| ATP + ADP | 1.3 | 0.7 |
| ATP + ADP + AMP | 1.3 | 0.5 |
| GTP | 1.9 | 0.7 |
| GTP + GDP | 1.7 | 1.1 |
| GTP + GDP + GMP | 1.2 | 0.8 |
| UTP | 1.5 | 0.9 |
| UTP + UDP | 1.2 | 0.6 |
| UTP + UDP + UMP | 1.4 | 0.8 |
| CTP | 1.7 | 0.9 |
| CTP + CDP | 1.5 | 0.9 |
| CTP + CDP + CMP | 1.5 | 1.0 |
The mean and SD represent CVs from 20 independent triplicate measurements from the liver ischemia time series (Figure 6). The reaction volumes were 10 μl (5 μl sample and 5 μl assay reagent).