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Gating strategies for T cell sorting
(A) Gating strategy for the identification and isolation of CD4+ total memory cells starting from CD4+ cells enriched by magnetic sorting.
(B) Gating strategy for the identification and isolation of in-vitro stimulated antigen-specific (CTV– CD25hi ICOShi), bystander-activated (CTV– CD25– ICOS–), and non-specific (CTV+ CD25– ICOS–) CD4+ T cells.
