| qPCR reaction master mix | ||
|---|---|---|
| Reagent | Final concentration/Amount | Amount |
| Fast SYBR Green Master Mix 2× | 1× | 10 μL |
| Fwd + Rev primer mix (10 μM) | 0.1 μM | 0.2 μL |
| Nuclease-free H2O | 7.8 μL | |
| Total volume | 18 μL | |
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