Lysis buffer

Reagent	Final concentration	Amount
Nuclease-free H2O		95.5 μL
10% (v/v) Triton X-100	0.2% (v/v)	2 μL
(40 U/μL) RiboLock RNase inhibitor	1 U	2.5 μL
Total		100 μL

Prepare a larger stock solution without the RNase inhibitor. Filter through a 0.22 μm PES membrane. Store at 4°C for up to 3 months. Add the RNase inhibitor to the working aliquot just before use.