RT Mix 1 (1 sample) | ||
---|---|---|
Reagent | Final concentration | Amount |
dNTPs (10 mM) | 0.5 mM | 1 μL |
Oligo(dT)20 (10 μM) | 0.25 μM | 0.5 μL |
SMART-TRAC oligo (10 μM) | 0.25 μM | 0.5 μL |
SMART-TRBC oligo (10 μM) | 0.25 μM | 0.5 μL |
Total volume | 2.5 μL |
RT Mix 1 (1 sample) | ||
---|---|---|
Reagent | Final concentration | Amount |
dNTPs (10 mM) | 0.5 mM | 1 μL |
Oligo(dT)20 (10 μM) | 0.25 μM | 0.5 μL |
SMART-TRAC oligo (10 μM) | 0.25 μM | 0.5 μL |
SMART-TRBC oligo (10 μM) | 0.25 μM | 0.5 μL |
Total volume | 2.5 μL |