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. 2023 Dec 21;5(1):102787. doi: 10.1016/j.xpro.2023.102787
RT Mix 1 (1 sample)
Reagent Final concentration Amount
dNTPs (10 mM) 0.5 mM 1 μL
Oligo(dT)20 (10 μM) 0.25 μM 0.5 μL
SMART-TRAC oligo (10 μM) 0.25 μM 0.5 μL
SMART-TRBC oligo (10 μM) 0.25 μM 0.5 μL
Total volume 2.5 μL