Figure 4.

DynROIs reveal the behavior of molecular adhesions in 3D environments

(A) Dual-colored orthogonal MIP of osteocarcinoma cells expressing eGFP-labeled paxillin and embedded in collagen labeled with Alexa Fluor 568. Overlay highlights dynROI.

(B) View of the dynROI.

(C and D) Detection of adhesions colored as a function of the degree of collagen contact and elongation.

(E) Probability density of elongation for adhesions with high and low degree of contact with collagen fibers (n = 1 cell).