Figure 1.

Group 1 ILCs are rare in the skin of naive mice and expand after local infection

(A) Representative FACS plots showing CD45⁺ live (top) and Lin⁻NK1.1⁺ gated cells (bottom) in the indicated organs.

(B) Absolute numbers of CD49a⁺NK1.1⁺ NK cells.

(C) Representative FACS plot showing Eomes⁺ NK cells in naive ear skin after intravascular labeling with anti-CD45 (ivCD45).

(D, E, and I) Numbers (D and I) and representative gating (E) of Lin⁻NK1.1⁺ cells in ear skin of mice infected with vaccinia virus expressing m157 (VACV) or S. aureus at the indicated times post infection.

(F and J) Confocal immunofluorescence images of skin cryosections from naive or VACV-infected mice housed in SPF (F) or wildling conditions (J). Scale bars represent 20 μm, 30 μm, or 50 μm, as indicated.

(G and H) Numbers of Eomes⁺ NK cells in the skin of individual ears on day 30 pi of B6 mice with WT or m157-expressing vaccinia virus (G) or of B6 versus Ly49H-deficient (Klra8^−/−) mice with VACV (H).

Data in (B), (D), and (I) were pooled from n = 6–8 mice (B), n = 7–23 ears (D), or n = 4–17 ears (I) per organ or time point. Data in (A), (C), (E)–(H), and (J) are representative of three independent experiments with n = 3–5 mice (A) or n = 3–6 ears (C, E–H, and J) per group. Error bars indicate mean + SD. p values were calculated by unpaired Student’s t test. ^∗∗p < 0.01 and ^∗∗∗∗p < 0.0001. n.s., not significant. See also Figure S1.