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. 2023 Dec 19;12:e85096. doi: 10.7554/eLife.85096

Figure 2. Proteomics identifies a serpinA1-like protein as the main pumiliotoxin (PTX)-binding protein in Oophaga sylvatica plasma.

(A) Streptavidin blot of the proteins pulled down from O. sylvatica plasma across the three conditions: no photoprobe, photoprobe, and photoprobe plus competitor PTX. (B) Quantitative proteomics output in terms of percent competition defined as 100% − average spectral counts in the photoprobe + PTX condition divided by average spectral counts in the photoprobe only condition. Average was taken across two replicates. SerpinA1-like protein and albumin are annotated. (C) The number of spectral counts across conditions for the serpinA1-like protein, each replicate is shown individually. (D) The number of spectral counts across conditions for the albumin protein, each replicate is shown individually.

Figure 2—source data 1. Raw data for the blot and proteomics shown in Figure 2.

Figure 2.

Figure 2—figure supplement 1. Peptide coverage over alkaloid-binding globulin (ABG) protein sequence.

Figure 2—figure supplement 1.

All unique peptides from one replicate of the proteomics were mapped onto the protein sequence of O. sylvatica ABG, showing that there were peptides covering the full length of the protein.
Figure 2—figure supplement 1—source data 1. Raw data for the proteomics shown in Figure 2—figure supplement 1.