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. 2023 Dec 11;12:e89822. doi: 10.7554/eLife.89822

Figure 7. Activin A promotes intramembranous bone repair of unicortical drill holes.

Figure 7.

(A) Representative sagittal (top) and transverse (bottom) cross-sections of micro-computed tomography (μCT) images of Activin A blocking antibody (nActA.AB)-treated drill-hole defects. Mice received injections of control IgG2b isotype or neutralizing monoclonal antibody against Activin A (nActA.AB, 10 mg/kg) twice a week after drill-hole injury. Arrows point to the defect region. Scale bar, 1 mm. (B) Bone volume fraction of intramedullary and cortical defect regions at days 7 and 21 post-injury. n = 4–6 mice/group. (C) Representative sagittal (top) and transverse (bottom) cross-sections of μCT images of recombinant Activin A (Act)-treated drill-hole defects. Two-month-old mice were implanted with a 50-μl Matrigel aliquot containing vehicle (Veh) or Activin A (Act) (1 μg) at the drill-hole site immediately after surgery. Arrows point to the defect region. Scale bar, 1 mm. (D) Bone volume fraction of intramedullary and cortical defect regions at days 7 and 21 post-injury. n = 4–6 mice/group. Data are expressed as means ± SD and analyzed by unpaired two-tailed t-test.

Figure 7—source data 1. Source data for Figure 7B.
Figure 7—source data 2. Source data for Figure 7D.