Table 5.

Challenges and limitations of HTS in clinical practice: key points.

Multiple dominant T-cell clones may be detected with unclear clinical significance.

Intraclonal CDR3 variants pose a challenge to clinicians because of ambiguous clinical significance.

Cost and lack of insurance coverage may be prohibitive to widespread adoption of HTS-TCR.

Quantity of tissue or amount of DNA probed may impact representation of gene segments.

Unable to distinguish between α/β or γ/δ chains of a specific TCR or whether a T-cell clone is CD4+ or CD8+

May not detect dominant clone in early-stage MF, making other factors like clinical presentation and histology important