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a, in vitro agar construct with two distinct phases - saline (clear) and agar (blue). DBS lead is placed in saline phase. b, DBS lead is fixed at either uniform saline or interface conditions with microactuator rig. c, A 15 s in vivo recording from Activa PC+S™ without active stimulation. d, A 15 s in vivo recording with active stimulation. Recording settling time was seen in first few seconds. e, Stimulation onset during voltage sweep shows stimulation transient.
