Skip to main content
. 2023 Dec 22;34:138–149. doi: 10.1016/j.bioactmat.2023.12.010

Fig. 3.

Fig. 3

In vivo therapeutic effect of intraperitoneally (i.p.) injected M-Exo/siR against colitis. (A) Treatment schedule of dextran sulfate sodium (DSS) colitis induction and i.p. injection of M-Exo/siR. After the acclimatization period, 8-week-old female BALB/c mice were i.p. injected with M-Exo/siR four times at three-day intervals beginning one day before 2.5 % DSS administration. (B) Daily body weight loss of mice. Data are mean ± SD (n = 8); ns = not significant, **p < 0.01, ****p < 0.0001. (C) Daily disease activity index (DAI) score. Data are mean ± SD (n = 8); ns = not significant, **p < 0.01, ****p < 0.0001. (D) Representative extracted colon image and colon length on day 28. Data are mean ± SD (n = 8); ns = not significant, *p < 0.05, ***p < 0.001. (E) Serum TNF-α cytokine level on day 28. Data are mean ± SD (n = 4); ns = not significant, **p < 0.01, ****p < 0.0001. (F) Tnfa mRNA level in colitis colon tissue on day 28. Data are mean ± SD (n = 4); ns = not significant, **p < 0.01. (G) Representative histopathological images of colon tissues stained with hematoxylin and eosin (H&E) on day 28. Open black circles (loss of surface epithelium); black arrows (infiltration of inflammatory cells); black triangles (ulceration); open black triangles (loss of goblet cells).