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. 2024 Jan 11;15(1):34. doi: 10.1038/s41419-023-06406-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B IHC staining of immune cells in ICC tumors developed in SPC mice. CD45 indicates total immune cells, CD3 indicates T cells, and F4/80 indicates macrophages. C Statistical analysis of immune markers in tumors, each dot indicates one mouse. sgSmad4 was included as a negative control. D, E IHC staining and statistical analysis of immune-related molecules in ICC tumors developed in SPC mice. ns: not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar = 25 μm.