Fig. 7.

HAI-1 expression correlates with cetuximab sensitivity and HAI-1 addition overcomes HGF-induced cetuximab resistance. A Correlation analysis of SPINT1 DNA methylation with its expression in CRC tumor samples. B SPINT1 DNA methylation comparison between normal and tumor. C Survival analysis (disease-free survival) in TCGA database based on low vs high SPINT1 DNA methylation. D Correlation between SPINT1 expression and response to cetuximab (CTX) in NCI-60 cell lines. E Two thousand CC-HGF cells were seeded in type I collagen and incubated with CTX (3 μg/ml) or HAI1 (10 μg/ml) alone or in combination as indicated for 14 days. Colony counts are plotted as mean ± SEM. F One hundred thousand CC and CC-HGF cells were cultured in type I collagen for seven days and incubated with CTX (3 μg/ml) and/or HAI-1 (10 μg/ml) for 48 h and then lysed and resolved on SDS-PAGE followed by immunoblotting for proteins as indicated