Fig. 1.

Light microscopy of primary microglia and exposure towards OGD with subsequent NEAT1 expression and cell viability analyses. Primary microglia were isolated from cerebral cortices and hippocampi of newborn WT C57BL/6 J mice. a Light microscopy of primary microglia in culture. b The magnified image of primary microglia under bright-field microscopy. c, d The corresponding NEAT1 expression at different time points of OGD and reoxygenation in comparison to PPIA. e, f The MTT assay was used to evaluate the cell viability after various OGD and reoxygenation time points. All results are expressed as mean ± standard deviation and analyzed by one-way ANOVA followed by Tukey's post-hoc-test. NS, no significance, **p < 0.01, ***p < 0.001, ****p < 0.0001. Abbreviations: NEAT1, nuclear paraspeckle assembly transcript 1; OGD, oxygen-glucose-deprivation; PPIA, peptidylprolyl isomerase A