Fig. 4.

Regulation of autophagy affects PLIN2 expression in primary microglia exposed to OGD. a, b The impact of autophagy on microglia survival after OGD was evaluated using different concentrations of the autophagy inhibitor 3‐MA or autophagy stimulator RAPA in comparison to microglia treated with the solvent DMSO using the MTT assay. c, d Relative PLIN2 expressions in microglial cells are shown after inhibition (c) or activation (d) of autophagy under OGD conditions using qRT-PCR normalized to PPIA. e–h Protein levels of PLIN2 in microglia are displayed after inhibition or activation of the autophagy pathway under OGD conditions. PLIN2 protein was recognized as a 48 kDa band, and GAPDH (36 kDa) was used as a reference. All results are expressed as mean ± standard deviation and analyzed by one-way ANOVA followed by Tukey's post-hoc-test. NS, no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Abbreviations: ASO, antisense oligonucleotide; OGD, oxygen-glucose-deprivation; 3-MA, 3-methyladenine; RAPA, rapamycin; PLIN2, perilipin 2; DMSO, dimethyl sulfoxide; PPIA, peptidylprolyl isomerase A