Fig. 5.

Regulation of autophagy affects LD formation in primary microglia. a Microglial cells were treated with 3-MA or RAPA under normoxia conditions, followed by BODIPY staining. b Microglia were treated with 3-MA or RAPA with additional ASO scramble or ASO NEAT1 treatment under hypoxia conditions, followed by BODIPY staining. c, d Quantification analysis of the percentage of LD in whole cells. All results are expressed as mean ± standard deviation and analyzed by one-way ANOVA followed by Tukey's post-hoc-test. NS, no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Abbreviations: LD, lipid droplets; 3-MA, 3-methyladenine; RAPA, rapamycin; ASO, antisense oligonucleotide; NEAT1, nuclear paraspeckle assembly transcript 1